정상 치은 조직에서 배양된 각화세포에서 Cyclosporine A에 의한 MMP-1, TIMP-1, TGF-β1의 발현에 관한 연구
THE mRNA EXPRESSION OF MMP-1, TIMP-1, TGF-β1 IN GINGIVAL KERATOCYTES FROM GINGIVAL HYPERPLASIA INDUCED BY CYCLOSPORINE A
강학수, 황경균, 심광섭, 빙정호, 박창주, 이재선, 임재중,
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강학수 ( Kang Hag-Soo ) - 한양대학교 의과대학 치과학교실 구강악안면외과
황경균 ( Hwang Kyung-Gyun ) - 한양대학교 의과대학 치과학교실 구강안면외과
심광섭 ( Shim Kwang-Sub ) - 한양대학교 의과대학 치과학교실 구강안면외과
빙정호 ( Bing Jung-Ho ) - 한양대학교 의과대학 치과학교실 구강안면외과
박창주 ( Park Chang-Joo ) - 한양대학교 의과대학 치과학교실 구강안면외과
이재선 ( Lee Jae-Sun ) - 한양대학교 의과대학 의생명연구소
임재중 ( Im Jae-Jung ) - 한양대학교 의과대학 치과학교실 치과교정과
KMID : 0355620080340040405
Abstract
Purpose: Cyclosporine A (CsA) is a versatile immunosuppresive agent used to prevent graft rejection syndrome and treat autoimmune disease. One of the major side effects associated with CsA is the abnormal gingival hyperplasia. The purpose of this study was to investigate the relationship between the mRNA expression of the MMP-1, TIMP-1, and TGF-β1 and the concentration of CsA in cultured human gingival keratinocytes.
Materials & Methods: Gingival keratocytes were obtained from gingival tissues of 4 healthy donors. The cultured gingival keratocytes were incubated with increasing concentrations of CsA (0-2000 ng/ml) for 24 hours and the expression of MMP-1, TIMP-1, and TGF-β1 were determined by reverse transcription-polymerase chain reaction (RT-PCR).
Results: The expressions of MMP-1 and TGF-β1 were not significantly different according to the concentrations of CsA. The expression of TIMP- 1 was significantly increased at the CsA concentration of 500 ng/ml.
Conclusion: We concluded that the gingival hyperplasia induced by CsA was more related with TIMP-1 than MMP-1 or TGF-β1 on gingival collagen metabolism in patients treated with CsA.
키워드
MMP-1;TIMP-1;TGF-β1;cyclosporineA;gingivalkeratinocyte
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